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Geochemical Journal
Geochemical Journal An open access journal for geochemistry
Published for geochemistry community from Geochemical Society of Japan.

Estimation of methanogenesis by quantification of coenzyme F430 in marine sediments

Masanori Kaneko, Yoshinori Takano, Nanako O. Ogawa, Yuki Sato, Naohiro Yoshida, Naohiko Ohkouchi
Geochemical Journal, Vol. 50, No. 6, P. 453-460, 2016

ABSTRACT

Coenzyme F430 is the hydrocorphinoid nickel complex which acts as active site in methyl-coenzyme M reductase (MCR). The MCR-F430 complex catalyzes the last step of methanogenesis: reduction of methyl-coenzyme M to methane. Since F430 is a common enzyme for mathanogens, it can be a function-specific biomarker to estimate biomass and activities of methanogens in environmental samples. A recently developed high sensitive method for coenzyme F430 analysis and a further purification step revealed F430 concentrations at 69 and 88 meters below seafloor (mbsf) off Shimokita Peninsula (529 and 31.3 fmol g-wet–1) and at 60 and 275 mbsf at Nankai Trough (31.4 and 26 fmol g-wet–1). Estimated methanogenic biomass and activities based on reported F430 content and activities in methanogens and prokaryotic cell weight, were ranging from 3.9 × 106 to 2.7 × 106 cells g-wet–1 and from 1.7 to 11 μmol d–1 g-wet–1.

KEYWORDS

coenzyme F430, methane hydrate, methanogens, function-specific compound analysis, marine sediment

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